Prayer space that was part, as was customary at the time, of the rooms of Duchess Anna, mother of King D. João IV.

The ceiling, executed in 1602-3 by the painter Tomás Luis, is decorated with frescoes of Italian inspiration that include vegetal elements and profane figures, called brutish, alongside representations of Santa Catarina and Santa Apolónia. From the same period, a series of paintings on wood by Frei Marcos da Cruz - scenes from the Life of the Virgin - a set that once adorned the Chapel of Colégio dos Reis Magos, the music school that operated until 1834 in a building adjacent to the Palace. On the wall that would correspond to the altar is a canvas painting by the French painter Pierre Antoine Quillard (1701-1733), representing the Annunciation.